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Thickness Correction

The thickness correction algorithm removes pathlength variations from spectra. The user needs to select a peak and a baseline. The algorithm offers various methods, please review the chapter thickness correction in the mathematics section for details.

The general thickness correction workflow consists of selecting the thickness band baseline and the actual thickness band. The thickness band baseline line will then be corrected by a two-point baseline algorithm with the selected parameters and the thickness correction will be performed. Selecting the parameters is performed as follows:

Thickness correction menu command​

To apply thickness correction using the menu command, please follow the instructions below:

  1. Activate the 2D data object you like to correct.

  2. From the Mathematics menu, select the Thickness correction command from the Spectrum Arithmetics submenu.

  3. In the Mathematics tab, select the parameters for thickness band baseline and the thickness band by entering numerical values.
     

  4. Alternatively, adjust parameters by using the vertical selection lines or rectangle areas in the spectrum view.

What are different selection tools good for?

Distinct vertical lines select single points in the spectrum to define peaks or the borders of peak areas. Colored rectangles define a range of points from which the actual anchor point will be calculated by the selected method (average, minimum, maximum). Please review details in the chapter thickness correction in the mathematics section.

  1. Click the Calculate button.

Thickness correction keyboard shortcut​

None.

Thickness correction parameters​

Please review the chapter thickness correction in the mathematics section for a full description of all parameters.

Parameters for selecting the thickness band:

Normalization​

By Intensity​

The user needs to select a single peak intensity for normalization.

By Spectrum Area​

The whole spectrum area is used for normalization.

By Peak Area​

A peak area is used for normalization. The peak area can be selected by different methods analog to the two-point algorithm selection methods.

Peak area calculation method​

The user needs to select the appropriate method for the peak area calculation. Options are:

  • Trapezoid

  • Algebraic sum

  • Absolute sum

Parameters for the thickness band baseline correction using the two-point algorithm:​

Method​

Please refer to the thickness correction chapter for details. Options are:

  • Single

  • Average

  • Maximum

  • Minimum

StartX, EndX​

StartX and EndX define the upper and lower limit of a peak area.

Start Minimum, Start Maximum​

Defines a region of points for the lower limit from which the actual anchor point will be calculated by the above selected method.

End Minimum, End Maximum​

Defines a region of points for the upper limit from which the actual anchor point will be calculated by the above selected method.